Distribution of the causative agent of relapsing tick-borne fever <i>Borrelia miyamotoi</i> in natural focus in the Tomsk region

BACKGROUND: Knowledge about the infectious potential of causative agent recurrent tick-borne fever Borrelia miyamotoi and role this pathogen in etiological structure formation transmissible natural focal infections a number regions remains incomplete. Among them is Tomsk region where 2021 incidence ixodic borreliosis was 15.7 per 100,000 population, which 3.6 times higher than all-Russian indicator.
 AIMS: This study aimed to summarize analyze epidemiological data, as well results our own clinical observations laboratory studies conducted 20152021 confirming distribution relapsing B. region.
 MATERIALS AND METHODS: Epidemiological data on manifestation foci were analyzed for period 20152021. A case febrile infection caused by described an example underdiagnosis fever. Verification variant carried out using real-time PCR. DNA extraction detection genetic markers performed RealBest series kits (Vector-Best, Novosibirsk), followed nucleic acid sequencing 23SrRNA, glpQ, recA gene sequences. Ixodid ticks (Ixodes persulcatus, Ixodes pavlovskyi, Dermacentor reticulatus) collected from studied infestation with miyamotoi. The pathogenic samples isolated tick homogenates Novosibirsk).
 RESULTS: In 2016, first confirmed molecular methods region. During 20152021, rate genus up 7%, ― 3%.
 CONCLUSION: demonstrated region, requires further monitoring epizootic situation transmission infections.